Regeneration of chrysanthemum (Chrysanthemum morifolium) via somatic embryogenesis and screening of clones for agronomic traits

Chrysanthemum morifolium propagation through stem cutting produces weak plants that show delayed anthesis above 20 °C and a reduced flower diameter. Therefore, in this study chrysanthemum plantlets were produced somatic embryogenic from calli to exploit the somaclonal variation for their improvement. Various explants variety Dante yellow cultured on Linsmaier Skoog (LS) medium with various concentrations of Kinetin (KT) 2,4-dichlorophenoxyacetic acid (2,4-D) combinations callus induction. Embryogenic proliferated regenerated by using different plant growth regulators. Regenerated acclimatized evaluated agronomic characteristics compared mother replicated field trial. Results revealed young leaf LS containing 9.02 µM 2,4-D 11.61 KT gave greater sizes as other treatments. A combination 0.44 6-benzylaminopurine (BAP) 5.37 1-naphthaleneacetic (NAA) yielded highest proliferation (0.27 g ± 0.03). significant number shootlets (25 0.8) was observed 0.45 2,4-D. During experiment, Clone S84 showed considerable improvement size found be promising clone commercialization. Somaclonal can used crop instead induced mutagenesis. Present substantial improved diameter via embryogenesis. Field screening increase flowers colour population. Both features are appealing consumers floriculture industry.